Additional file 1: of Swift metabolite changes and leaf shedding are milestones in the acclimation process of grapevine under prolonged water stress

Table S1. Stomatal conductance measurements of 2014 and 2015 presented as mean (N = 4) and standard error. The respective time points are indicated with days from treatment application. Table S2. Fold change (Treatment/Control) of leaf secondary metabolites during the time course of the experiment in 2014 and 2015. Table S3. Fold change (Treatment/Control) of leaf central metabolites during the time course of the experiment in 2014 and 2015. Table S4. Summary of RNA sequencing analysis metrics. The table show the number of filtered reads and aligned reads (filtered) to the 12X grapevine reference genome that are counted. Table S5. Transcript abundance of the all 29,970 genes, reported as Reads Per Kilobase of transcript, per Million mapped reads (RPKM), in leaves of well-watered and water deficit treated grapevines at day 4, 8 (2014) and day 12 (2015). Tables S6–S8. Summary of differentially expressed genes (FDR < 0.05, |log2 FC| > 1) in grapevine leaves comparing well-watered and water deficit treatments. DESeq2 statistical outputs of day 4, 8 (2014) and day 12 (2015) treatments are listed in Table S6, S7, and S8, respectively.. Annotation of genes were referred from Vitisnet ( https://www.sdstate.edu/agronomy-horticulture-and-plant-science/functional-genomics-bud-endodormancy-induction-grapevines-5 ) (Grimplet et al. 2009). Table S9. List of differentially expressed genes in grapevine leaves that are common and unique leaves in each intersection of the Venn diagram described in Fig. 4b. Table S10. Summary of enriched (FDR < 0.05) plant GO slim categories in grapevine leaves comparing well-watered and water deficit treatments at day 4, 8 (2014) and day 12 (2015). Other statistical outputs of BiNGO (Maere et al. 2005) are reported. Table S11. Summary of cis-regulatory elements (CRE) enriched at a threshold of FDR < 0.01 in promoters of drought-regulated genes at day 4, 8 (2014) and day 12 (2015). For each treatment group, the number of genes containing the CRE, group size, total number of genes in the genome containing the CRE, promoter size, enrichment P-value, FDR, and lists of genes containing the CRE with their CRE position along the gene promoter are reported. (XLSX 3596 kb)