RNA-seq, term-seq and tagRNA-seq of M. tuberculosis strains H37Rv and RhoDUC strains induced for Rho depletion at times 0H, 1.5H, 3H, 4.5H and 6H

Accurately controlled termination of transcription is critical for gene expression and adaptation to changing environments in bacteria. The aim of this study was to define transcription termination sites in Mycobacterium tuberculosis at nucleotide resolution. To this end, we used a combination of term-seq to identify termination of transcription and tagRNA-seq to identify transcription start sites and processed sites. This led us to identify a prevalence of Rho-dependent termination of transcription in the pathogen. We applied term-seq and WTSS RNA-seq on the RhoDUC strain derived from H37Rv, for which a depletion of Rho is inducible by addition of Anhydrous Tetracyclin, to identify Rho-responsive termination site and quantify readthrough.