Benchmarking ploidy estimation methods for bulk and single-cell whole genome sequencing

To evaluate the ability of ploidy estimation tools to detect complete polyploidy, we induced whole-genome duplication through mitotic slippage in RPE1 cells, which are hTERT-immortalized retinal pigment epithelial cells, and then sorted the cells to generate WGS data by mixing diploid cells with an equal amount of tetraploid or octoploid cells.