Fecundity of diamondback moth females when offered honey with pyridalyl, a selective insecticide

We previously reported that the presence of pyridalyl, a selective insecticide, in aqueous honey  (50 % v/v) negatively affects the survival of diamondback moth (Plutella xylostella: DBM) adults. However, it remains unclear whether toxicity of pyridalyl in aqueous honey affects the fecundity of adult female DBMs. We analyzed the survival and fecundity of adult DBM under the water, honey, or honey + pyridalyl conditions in glass tubes containing Japanese mustard spinach (Brassica rapa: Komatsuna) leaves for 10 days. The survival of adults under the honey (50 % v/v) and honey + pyridalyl (100-fold dilution) conditions was significantly higher and lower, respectively, than under the water condition during the experimental period. The number of eggs laid by DBM females each day (fecundity) under the water condition was significantly lower than that under the honey condition throughout the experimental period, except on day 1. In contrast, the numbers under the water, and honey + pyridalyl c..., Food sources
A commercial formulation of pyridalyl (Pleo® flowable: pyridalyl 10 % in water; Sumitomo Chemical Company Limited, Osaka, Japan) was used as the selective insecticide. A 100-fold dilution of pyridalyl in aqueous honey solution was selected as the test food source (see Introduction). An aqueous honey solution (50 % v/v) was provided to DBM adults as a positive control food source. As a negative control, we offered a non-sugary food source (water only) to DBM adults.
Experiments
Experiments were conducted in glass tubes and acrylic cages. Glass tube experiments were designed to isolate and examine the effects of pyridalyl on honey. Acrylic cage experiments were structured to evaluate how the combination of plants and pyridalyl in honey affected the performance of DBM adults.  
Glass tube experiment. The effects of different food sources on the survival and number of eggs laid by DBM females were observed in a glass tube (1.8 cm diameter; length: 12 cm) containing a piece of ..., , # Fecundity of diamondback moth females when offered honey with pyridalyl, a selective insecticide

[https://doi.org/10.5061/dryad.q573n5tq3](https://doi.org/10.5061/dryad.q573n5tq3)

Data contents:
Figure 1. (a) Survival curves of female diamondback moths maintained under the water, honey, and honey + pyridalyl conditions in a glass tube for 10 days. (b) The relative number of adults survived on the three experimental day under the water, honey, and honey + pyridalyl conditions in an acrylic cage.
Figure 2. Number of diamondback moth eggs per day in the glass tube.
Figure 3. (a) Total number of diamondback moth eggs in the glass tube during day 10. (b) Numbers of diamondback moths (larvae and pupae) in an acrylic cage.