Aryl Hydrocarbon Receptor signaling in response to 30 oil sub-fractions

This is a lacZ reporter gene bioassay that measures aryl hydrocarbon receptor signaling in response to aromatic hydrocarbons. Here, fractions from the Overton laboratory prepared on 1-20-2017 (13 sub-fractions) and 1-26-2017 (17 sub-fractions) were tested at 3 dilutions for bioactivity. The oil sub-fractions were compared by % normalization to signaling of the positive reference ligand beta-naphthoflavone which was set to 100%. Signaling at greater dilutions indicates greater potency and/or greater chemical content in the sub-fractions.