Transcriptome and mRNA stability analysis of neuronal differentiation
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https://www.ncbi.nlm.nih.gov/sra/SRP345503
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资源简介:
RNA-seq and mRNA stability assay were used to probe changes occuring neuronal differentiation and maturation of SH-SY5Y cells. We analyzed differentially expressed genes after neuronal maturation via RNA-seq. We also analyzed mRNA stability by utilizing Actinomycin D mediated transcription blockage followed by RNA-seq. Libraries were PolyA enriched for mRNA and prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.
创建时间:
2025-02-18



