Genome re-sequencing of Arabidopsis epiRILs and epiHybrids

Despite the importance and wide exploitation of heterosis in commercial crop breeding, the molecular mechanisms behind this phenomenon are not completely understood. In this study we aimed to understand and quantify the contribution of DNA methylation variation to heterosis in various traits of Arabidopsis. Therefore we created epigenetic hybrid lines from ddm1-derived epiRILs. We performed whole genome sequencing on four epiHybrids and their parental lines (epiRILs & Col-wt) in duplicates to examine structural variants in hybrids genome wide and with a focus on previously mapped QTL regions. Overall design: WGS is performed in four epiRIL lines and four epiHybrid lines (epiRILs 92, 150, 193, 232 and epiHybrids 92H, 150H, 193H, 232H and Col-wt)