Effect of TWIST1 dosage changes on chromatin accessibility in hESC-derived cranial neural crest cells

To assess the effects of quantitative TWIST1 dosage changes on chromatin accessibility in hESC-derived cranial neural crest cells, we performed genome editing of the H9 hESC lines to tag TWIST1 with FKBPV36 and V5, Upon differentiating edited hESC to cranial neural crest cells using an established protocol, addition of differing concentrations of the degrader molecule dTAGV-1 results in different TWIST1 concentrations, as measured by V5 intracellular staining and flow cytometry. We then profiled chromatin accessibility in each of these TWIST1 concentrations Genome-wide chromatin accessibility profiling (ATAC-Seq) of TWIST1-tagged H9 hESC-dervied cranial neural crest cells (CNCCs)