Gene expression data of organoids derived from traditional serrated adenomas. Gene expression data of organoids derived from traditional serrated adenomas

Traditional serrated adenomas (TSAs) are rare colorectal polyps characterized by unique histology features. Fusions in R-spondin genes have been found in TSAs, but it is not clear whether these are sufficient for TSA development. We established 2 patient-derived TSA organoid lines, and engineered chromosome rearrangements that involve R-spondin genes into human colonic organoids using CRISPR-Cas9. Consequently, we highlighted the similarity of the gene expression patterns between TSA organoids and engineered organoids. Overall design: We analyzed gene expression of 2 patinet-derived traditional serrated adnoma, 3 genetically engineered and 1 normal colonic organoids. Total RNA were extracted from organoids and hybridized to GeneChip PrimeView Human Gene Expression Array, and scanned with GeneChip Scanner 3000 7G.