Next Generation Sequencing profile of leukemia from distinct cells-of-origin [Transcriptome profiles]
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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http://data.iscr.ac.cn/Article?id=72ff264a9b87b9301e22a460f353f689
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The goal of this study is to identify genomic signatures predicitve of cell-of-origin in acute myeloid leukemia50K bulk leukemia (GFP+) cells from the spleen of recipient mice were sorted directly into 350μl of RLT buffer (Qiagen) and flash-frozen. Total RNA was isolated according to manufacturerâs protocols (Qiagen) including DNase treatment, and quality was assessed using an Agilent 2100 Bioanalyzer and RNA 6000 Nano kit. Amplified cDNA was sheared to approximately 300bp using a Covaris E220 Focused Ultrasonicator. RNA-seq library preparation used the TruSeq DNA sample prep kit v2 (Illumina).Libraries were sequenced on the Illumina HiSeq 2000 platform.
提供机构：
The Jackson Laboratory
创建时间：
2022-02-20
