CRISPR deletion of ELAVL1 (HuR) in MIA PaCa-2 in vitro cells and in vivo tumors

To investigate the impact of the mRNA stabilty factor HuR on the pancreatic cancer transcriptome in MIA PaCa-2 in vitro cell line and in vivo tumors at multiple time points of tumor growth Overall design: CRISPR-mediated deletion of ELAVL1 (HuR) in MIA PaCa-2 PDAC cells. HuR wildtype (WT) or HuR knockout (KO) cells in culture (in vitro) were collected at 70% confuency for RNA extraction. HuR WT and HuR KO orthotopic tumors (in vivo) were harvested 2, 3, and 4 weeks post-injection for RNA extraction.