Prediction software analysis of novel missense mutations detected in the present study.

Polyphen-2 - score 0 to1 shows low to high confidence for probability of protein damaging.SIFT- mutations considered as pathogenic showing score Align GVGD- higher the score higher the probability of protein damaging nature of mutationMUpro - higher the negativity in SVM score higher the probability of decrease in protein structure stability.SVM- Support Vector Machine (SVM score – negative score for novel missense mutations showed the pathogenic probability).