Autonomous neutrophil ageing in the lung governed by the pulmonary microbiome

We used cellular indexing of transcriptomes and epitopes-based single cell RNA sequencing (CITE-scRNAseq) coupled with adoptive transfer approaches to determine tissue contribution to neutrophil diversity. Overall design: Bone marrow, blood, liver and lung were isolated from WT mice. Cells were hashed, and DAPI-Ly6G cells were FACS sorted from these tissues. Cells were then processed (10X chromium)