Water record of <em>Crassostrea angulata</em> transgenerational ocean acidification experiment

The basic observed water characteristics (pH, temperature, salinity and total alkalinity (TA)) and calculated water carbonate chemistry in the hatchery located in Zhanjiang, China (21.07°N, 110.74°E). The two-year-old Portuguese oysters <em>Crassostrea angulata</em> were collected from Wuchuan, China (21.37°N, 110.74°E) in 2020. To be specific, about 300 adult oysters were transferred into six black fibreglass tanks (500L each) randomly and each culture tank contained about 50 individuals. Six tanks were independent and completely randomized to be separated into two different pH levels: ambient pH 8.0~8.1 as control (named F0_8.1) and pH 7.4 as ocean acidification (OA) treatment (named F0_7.4) for about one month to produce F1 generation. The F1 larvae were acquired by artificial fertilization of matured F0 generation (F0_8.1 and F0_7.4). Embryos from F0_8.1 were separated into two pH levels: F1_8.1-8.1 and F1_8.1-7.4. Likewise, embryos from F0_7.4 were separated into two pH levels: F1_7.4-8.1 and F1_7.4-7.4. Thus, there were four treatments for F1 generation, and each treatment had three independent 500L tank replicates (A, B, and C). F1 generation continued to be reared to the next year (2021) till most of them reached the first year of gonad maturation to produce F2 generation. The F2 larvae were acquired by artificial fertilization of F1 generation. Embryos from F1_8.1-8.1 were separated into two pH levels: F2_8.1-8.1-8.1 and F2_8.1-8.1-7.4. The embryos from other F1 treatments were under similar processes. There were eight treatments for F2 generation and reared till 2022 to test the transgenerational OA effects on oyster biological responses. Low pH was driven by bubbling of CO₂-enriched air mixed with ambient air into filter seawater in the treatment tanks. pH and temperature were monitored daily with pH meter (Mettler Toledo InLab^®, Switzerland) calibrated with NBS standards. Salinity was monitored using a dual-scale refractometer (MingRui, China). The seawater was utterly changed every two days during larval stage and every week for juvenile stage. About 50 mL of seawater was preserved with HgCl₂ for total alkalinity (TA) measurement. The TA was measured using an alkalinity titrator (Mettler Toledo G20S Compact Titrator, Switzerland), and pCO₂, CO₃^2-, saturation states of calcite and aragonite were calculated by CO2SYS program with equilibrium constants K2 and KSO4 (Millero, F. J., Graham, T. B., Huang, F., Bustos-Serrano, H., &amp; Pierrot, D. (2006). Dissociation constants of carbonic acid in seawater as a function of salinity and temperature. Marine Chemistry, 100(1), 80-94. https://doi.org/10.1016/j.marchem.2005.12.001

本数据集涵盖中国湛江（21.07°N，110.74°E）某育苗场的基础观测水体理化特征（pH、水温、盐度及总碱度（total alkalinity, TA））与计算得到的水体碳酸盐化学参数。

2020年，研究人员从中国吴川（21.37°N，110.74°E）采集了2龄葡萄牙牡蛎<em>Crassostrea angulata</em>。具体实验流程如下：随机选取约300只成年牡蛎，均分至6个500L黑色玻璃钢水槽中，每个水槽放养约50只个体。6个水槽相互独立且完全随机分组，设置两种pH梯度：以环境pH 8.0~8.1作为对照组（命名为F0_8.1），以pH 7.4作为海洋酸化（ocean acidification, OA）处理组（命名为F0_7.4），培育约1个月以获得F0代亲本的F1子代。

通过对成熟F0代（F0_8.1与F0_7.4）进行人工授精获得F1幼虫。将来自F0_8.1的胚胎分为两组，分别设置为F1_8.1-8.1与F1_8.1-7.4；同理，将来自F0_7.4的胚胎分为两组，分别设置为F1_7.4-8.1与F1_7.4-7.4。由此F1代共设置4种处理，每种处理设置3个独立的500L水槽重复组（A、B、C）。

F1代持续培育至2021年，待多数个体达到性腺成熟的1龄阶段，以获得F2子代。通过对F1代进行人工授精获得F2幼虫。将来自F1_8.1-8.1的胚胎分为两组，分别设置为F2_8.1-8.1-8.1与F2_8.1-8.1-7.4；其余F1代处理组的胚胎均遵循相同的分组流程。F2代共设置8种处理，持续培育至2022年，以探究跨代海洋酸化对牡蛎生物学响应的影响。

低pH条件通过向处理水槽的过滤海水中通入CO₂富集空气与环境空气的混合气体实现。每日使用经NBS标准校准的pH计（梅特勒托利多InLab^®，瑞士）监测pH与水温；使用双刻度折光仪（明瑞，中国）监测盐度。幼虫培育阶段每2天完全更换一次海水，稚贝培育阶段则每7天更换一次海水。取约50mL海水，用氯化汞（HgCl₂）固定，用于总碱度（TA）测定。总碱度通过碱度滴定仪（梅特勒托利多G20S Compact Titrator，瑞士）测定；pCO₂、CO₃²⁻、方解石与文石饱和度状态则通过CO2SYS程序结合平衡常数K₂与KSO4计算得到（Millero, F. J., Graham, T. B., Huang, F., Bustos-Serrano, H., & Pierrot, D. (2006). 海水中碳酸的解离常数与盐度及水温的关系. 海洋化学, 100(1), 80-94. https://doi.org/10.1016/j.marchem.2005.12.001）

搜集汇总

背景与挑战

背景概述

该数据集记录了中国湛江孵化场中葡萄牙牡蛎跨代海洋酸化实验的水化学参数，包括pH、温度、盐度和总碱度等基本观测值及计算出的碳酸盐化学数据。实验通过控制不同pH水平（如环境pH 8.0~8.1和酸化pH 7.4）进行跨代研究，从F0代至F2代，旨在分析海洋酸化对牡蛎生物反应的长期影响。数据集提供了详细的实验方法和跨代处理信息，适用于海洋生态学和生理学研究。