An analysis of the effect of indacaterol and GSK 256066 alone and in combination on gene expression changes in the BEAS-2B human airway epithelial cell line.

Microarray analysis was performed on RNA extracted from BEAS-2B cells treated with indacaterol, GSK 256066 and indacaterol and GSK 256066 in combination to identify differentially expressed genes that may contribute to the beneficial and deleterious effects of β2-adrenoceptor agonists and phosphodiesterase 4 inhibitors in obstructive lung diseases. Confluent BEAS-2B cells were treated with vehicle, indacaterol (Ind; 10nM), GSK 256066 (GSK; 10nM) or Ind and GSK in combination for 1h, 2h, 6h and 18h. Total RNA was extracted, quantified (NanoDrop 2000) and the quality of each sample was determined by using the Agilent 2100 Lab-on-a-Chip system before being processed for gene expression chnages by Expression Analysis Inc (Dunham, NC, USA). Please note that the sample records have been updated with additional samples and re-accessioned with the re-processed data on March 14, 2018.