Supplemental Material for Espinoza and Berg, 2020

<b>Figure S1: </b>Egg chambers develop sequentially in the ovary. A) Schematic diagram of <i>Drosophila</i> ovaries depicting egg chambers developing progressively in assembly lines called ovarioles. Each ovariole contains egg chambers organized sequentially, with youngest at the anterior and most mature at the posterior. B) Schematic diagrams of three stages of egg-chamber development showing somatic follicle cells in white and germline-derived nurse cells and oocyte in light orange. Nuclei are represented by blue circles. Dorsal-appendage-tube lumen is shown in black in S12. C) Timing of <i>GAL4</i> expression. <i>CY2-GAL4</i> is expressed at stage S6 in all follicle cells, while <i>c415-GAL4 </i>is expressed at stage S10B only in the stretch cells; in both cases the expression continues to the end of oogenesis. <b>Figure S2: </b>Modifier Screen crosses used to identify and narrow down regions that suppress or enhance the <i>Idgf3-</i>overexpression phenotype. Chromosome IV is not shown. A) Cross to obtain the control females. Females from the <i>Idgf3-</i>overexpression stock, which was kept at 25^o for the main modifier screen and then kept at 22^o for narrowing down regions, were crossed to <i>w¹¹¹⁸</i> males. B) Cross to obtain the testing females. Females from the same <i>Idgf3-</i>overexpression stock from A were crossed to males from each stock of the 3L deficiency kit to obtain the females for testing; these females were identified by the lack of the Balancer chromosomes (which carry a dominant marker). <b>Figure S3: </b>Collage of representative eggs observed in the modifier screen, grouped into three categories: Normal/mild, moderately defective, and severely defective dorsal appendages (DAs). The DAs on normal eggs have a round paddle and flat stalk, with each making up ~ 50% of the length of the DA. In addition, the edges of the DAs are smooth, and the bases do not touch. Mild eggs had DAs that closely resembled those of normal eggs but had one slightly defective feature, such as marginally wider paddles or bases that were closer together. Eggs were classified as moderately defective when having wavy or serrated DA edges; some eggs had triangular-shaped DAs or DAs of increased size; some eggs had no clear separation between paddle and stalk. Eggs were classified as severely defective when they were half the length of a normal egg, or when the eggs were of normal length, but the DAs had chorion material that did not resemble normal DAs. For example, some eggs had chorion material in between the DAs, or the chorion material meant to form the DAs was in a pool on the dorsal anterior side of the egg. Some eggs in this category had DAs in which their stalks were completely fused.