Effect of FL-Fc-DM1 drug on gene expression of Molm-13 cells pretreated with cytarabine.

Cytarabine is one of the first line chemo drugs to treat acute myeloid leukemia (AML). However, cytarabine can induce AML cell cell cycle arrest at G0/G1 phase. These cell-cycle-arrested AML cells resist cytarabine, show a senescence-like phenotype or stem-cell like phenotype. To eliminate these cell-cycle arrested cells, we developed a FL-Fc-DM1 drug by conjugating FLT3 liangd Fc fusion protein and mertansine. We used the Molm-13 cells as a AML model. The total mRNA of Molm-13 cells treated with different drugs (cytarabine, FL-Fc-DM1) were sequenced. Overall design: Molm-13 cells were cultured in RPMI 1640 medium supplemented with 10% FBS. In the control group, Molm-13 cells were cultured for 4 days. In the cytarabine group, Molm-13 cells were treated with 0.5/1 µM cytarabine for 4 days. In the FL-Fc-DM1 treatment group, Molm-13 cells were treated by cytarabine (0.5/1 µM) for 3 days and then treated with FL-Fc-DM1 (1 nM) for another 24 hours in the absence of cytarabine. We than analyze the transcriptome differences between different groups.