Single cell RNA-seq data for mouse fallopian tube and ovary

Epithelial ovarian cancer (EOC) is the most lethal malignancy of the female reproductive system. In order to improve EOC patient outcomes, it is crucial to have a better understanding of how EOC develops from its cellular origin. EOCs can originate from either fallopian tube epithelial (FTE) cells or ovarian surface epithelial (OSE) cells, but with different courses of development. The basis for this difference is unclear. To address this, we performed single cell RNA-sequencing of mouse cells isolated from the distal portion of fallopian tubes (i.e., oviducts) and surface layer of ovaries. Analysis of this single cell dataset revealed distinct niche organizations for murine FTE cells and OSE cells. Fallopian tube and ovarian tissues from two wild-type adult FVB female mice were isolated. Single cells were prepared from the distal half (i.e., the portion close to ovary, thus including the fimbrial region) of fallopian tubes and from the stripped surface layer of each ovary. The resulting single cells were subjected to barcoding using the inDrop platform. The resulting expression libraries were then subjected to high throughput sequencing.上皮性卵巢癌（Epithelial Ovarian Cancer, EOC）是女性生殖系统中致死率最高的恶性肿瘤。为改善上皮性卵巢癌患者的临床预后，深入解析其细胞起源及癌变进程的调控机制至关重要。上皮性卵巢癌可起源于输卵管上皮（Fallopian Tube Epithelial, FTE）细胞或卵巢表面上皮（Ovarian Surface Epithelial, OSE）细胞，但二者的发育进程存在显著差异，其背后的分子基础尚未明确。 为解决这一科学问题，本研究对从小鼠输卵管远端（即输卵管段）及卵巢表层分离得到的细胞开展了单细胞RNA测序。通过对该单细胞数据集的分析，我们揭示了小鼠输卵管上皮细胞与卵巢表面上皮细胞各自独特的细胞龛（niche）结构。 本研究采集了2只野生型成年FVB品系雌性小鼠的输卵管与卵巢组织，分别从输卵管远端半段（即靠近卵巢的区段，包含伞部区域）及各卵巢剥离后的表层组织中制备单细胞悬液。随后采用inDrop平台对单细胞进行条形码标记，构建表达文库并开展高通量测序。