RNA sequencing of suprachiasmic nucleus (SCN) of mice with rev-erb??/?? knockout in GABAergic neuron

We compared differentially expressed genes (DEGs) in isolated SCN of female wild-type and GABAergic neuron rev-erb??/?? knockout (KO) mice at ZT12-14. 29 genes are significantly upregulated and 14 genes are statistically downregulated in SCN of KO mice. Aside from the circadian rhythm as an expected top-enriched functional entity, DEGs were enriched with cell surface proteins and associated modulators. Many of these genes are related to neurotransmission or neurological diseases. Overall design: SCN were isolated at ZT12-14. Equal amount of RNA from 2 SCN were pooled for each sample. Three samples were analyzed for each genotype.