LungART(IFCT-Trial)_TP_data

License: CC BY-NC 4.0 Overview This repository contains the RNA-sequencing and associated clinical data supporting the findings reported in our manuscript. The data are provided for academic and non-commercial use. RNA extraction was performed using the Promega Maxwell RSC RNA FFPE Kit. RNA libraries were generated using the SureSelect XT HS2 RNA Reagent Kit and sequenced in paired-mode on the Illumina NovaSeq 6000 platform in one batch. Molecular barcode (MBC) preprocessing was performed using Agilent AGeNT tools. Transcripts quantification was performed using Kallisto, and gene level TPM values were used for TP estimation via the ESTIMATE algorithm, from the corresponding R package. Contents File / Folder Description Format Rnaseq_txi.rds Processed RNA-seq expression data RDS (R format) – can be accessed in R using readRDS(); compatible with DESeq2 via DESeqDataSetFromTximport() FASQC_merged_report.html RNA-seq quality control summary HTML clinical_data.tsv Clinical metadata for the samples analyzed in the study TSV (tab-separated values) Data Access Data will be made publicly available upon acceptance of the manuscript. Requests for access prior to publication can be directed to the corresponding author.