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RNA expression in CCL19-treated CD4+ T cells

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下载链接:
https://www.ncbi.nlm.nih.gov/sra/SRP417153
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资源简介:
Transcriptome analysis of mouse naïve CD4+ T cells with or witout chemokine CCL19 treatment. Overall design: Purified naïve CD4+ T cells (1 million) were cultured in 200 microlitter complete RPMI1640 with immorbilized anti-CD3 and -CD28 antibodies (coating at 1 microgram/mL) in 96 well plate in the presence or absence of recombinant mouse CCL19 for 6 hours. Cells were harvested, RNA was extracted, and cDNA libraries were generated. Comprehensve gene expression was analyzed by RNA sequencing.
创建时间:
2023-03-31
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