Proteomic Profiling of HEK293-F cell and human peripheral blood neutrophil proteins extracted by conventional direct extraction and phenol-guanidinium isothiocyanate (P/GTC)-based extraction methods

To evaluate the performance of the phenol-guanidinium isothiocyanate (P/GTC)-based extraction method in proteomic analysis, we performed proteome analysis from HEK293-F cell and human peripheral blood neutrophil proteins extracted by the P/GTC-based extraction and conventional direct extraction method. Furthermore, the P/GTC extracted proteins were analyzed by 2D-LC-MS/MS.