mRNA profiles of hematopoietic stem cells from p5 neonate and 4 weeks old mice.

Purpose: The goals of this study are to elucidate the underlying charactristic difference of p5 neonatal and 4 weeks old hematopoietic stem cell. Methods: p5 neonatal and 4 weeks old 100 cells of HSCs were sorted. Moreover, after HSCs were cultured for 1day, 100 cells of phenotypic HSCs were sorted. These cells were subjected to mRNA sequence using Next-seq. The sequence reads that passed quality filters were analyzed by CLC genomic workbench. Overall design: p5 neonatal or 4 weeks old mice, HSCs (CD150+ CD48- EPCR+ Lineage-) were sorted were sorted, and subsequently mRNA profiles were generated by deep sequencing using Next-seq. After culturing HSCs (CD150+ CD48- EPCR+ Lineage-) derived from untreated mice for 1 day, mRNA profiles were generated by deep sequencing using Next-seq .