Genome analysis by next-generation sequencing of potent x Ler descendants

To gain further insight into the root clock mechanism, we performed a mutagenesis screen in plants carrying DR5::Luciferase in combination with markers for subsequent LR organogenesis (pWOX5::ER-YFP and pSCR::ER-GFP12). Out of this screening, we identified a mutant with increased expression of DR5::Luciferase in the OZ and PBS distributed throughout the primary root axis without evident spacing. We further refer to this mutant as potent. Approximately 200 seeds generated in the fifth cross of potent x Ler were sown and seedlings with (mutant) and without (Control) potent phenotype were collected and frozen at -80° C. The SNPtrack pipeline was used to identify single variant/nucleotide polymorphisms associated to Ler ecotype as well as all the heterozygous mutations present in the potent sample.