Transcriptomic Changes in Microdissected CCDs after Three Hours Lipopolysaccharide (LPS) Administration in Rats

Ureteral obstruction is marked by disappearance of the vasopressin-dependent water channel aquaporin-2 (AQP2) in the renal collecting duct and polyuria upon reversal. Most studies of unilateral ureteral obstruction (UUO) models have examined late time points, obscuring the early signals that trigger loss of AQP2. Here, we performed RNA-Seq on microdissected rat cortical collecting ducts (CCDs) to identify early signaling pathways post-UUO. To address whether the induction of inflammatory signaling is a cause of loss of AQP2 expression, we directly induced inflammatory signaling in rats with lipopolysaccharide (LPS) administration Cortical collecting ducts were microdissected from the kidneys 3 hours after intraperitoneal injection with lipopolysaccharide (LPS) or Vehicle (normal saline) (n=3 versus n=3, paired). Microdissected tubules were subjected to small sample RNA-Seq analysis as previously described (PMID: 31146973).