Characteristics of the IgG-secreting hybridomas obtained from immunization of Balb-c mice with native HIV-1 Env complexed with sCD4.

Binding activity to the indicated solid-phase antigens of IgG present in culture supernatants from hybridomas derived from spleen cells of mice immunized with NIH-3T3 cells infected with vaccinia recombinant vCB43, expressing Env from the R5 HIV-1 isolate Ba-L, and complexed with 2-domain sCD4. Binding activity was measured with culture supernatants diluted 1∶1 (see methods). Values are qualitative, since different microcultures contained different numbers of cells. Asterisks indicate supernatants where an increased binding to the 2D-CD4/gp120 (Ba-L) complex was observed as compared to the binding to each moiety separately.