Amplicon sequencing results for Alu/Asu-bt-B.

Excel spreadsheet of amplicon sequencing results for Alu/Asu-bt-B following analysis with the DADA2 v.1.11.5 bioinformatic software package and manual assessment for chimeras, off-target amplicons and PCR artefacts. The first column of the spreadsheet contains the single worm identification. In the adjacent columns the read number for each unique amplicon sequence variant (ASV) is given. From the dataset, 21 ASVs with extremely low reads (below 40) and only detected in 1 or 2 worms were manually deleted since these were seen as PCR artefacts. Furthermore, due to a lack of complete specificity of the primers, the dataset contained 10 ASVs corresponding to the Alu/Asu-bt-A locus, which were also manually deleted. The genotype per worm was defined by applying a threshold of 1,000 reads. ASVs below threshold level were excluded from further population genetic analyses and are indicated in red in the table. There was one A. lumbricoides worm with only reads below threshold and five A. suum worms with reads above threshold for more than two ASVs, these six worms were not included in the genotype data used for population genetic analysis. (XLSX)