Transcriptional profilling of female mice liver as a function of age

To study the ontogeny of pharmacological relevant genes, such as, drug targets, transporters and metabolic enzymes, six wild-type C57BL/6JCrl female mice were sacrified at ten different age points, in order to capture potential transcriptomic switches as the mice progresses from newborn to infant, from infant to teenager, from teenager to adult, and finally from adult to old-adult (P1 - 1 day old; 1W - 1 week old; 2W - 2 weeks old; 3W - 3 weeks old; 1M - 1 month old; 2M - 2 months old; 3M - 3 months old; 6M - 6 months old; 1Y - 1 year old; and 2Y - 2 years old). Liver, heart, kidney, lungs and brain were extracted from all 60 females in the study, and liver samples were processed for RNA extraction. Overall, 8.3% of pharmacological relevant genes changed their expression with age, of which 57% were metabolic enzymes, like Cyp2c29 and Fmo3, and 22% were transporters.