Influence of GPR35 deficiency on the ileal and macrophage transcriptome

Mutations in GPR35 (G protein coupled receptor 35) are linked to ulcerative colitis and primary sclerosing cholangitis. How GPR35, which interacts with the Na/K-ATPase to determine ion transport, influences the transcriptome of macrophages and the ileum is unknown. Here compare transcript expression in bone marrow-derived macrophages and isolated ileum from mice deficient in GPR35 (knockout) with wild type mice by bulk RNA sequencing. Overall design: Ileal tissue from wild type (n=5) and GPR35-deficient mice (n=5) was isolated and RNA extracted. Bone marrow-derived macrophages were cultured from wild type (n=5) and GPR35-deficient (n-5) mice and RNA extracted as described previously.