#Restriction enzyme XmaI sites are in italics and underlined. *Point mutation sites are in bold and underlined in primer sequence NS2-F, NS3-F and NS4-F.
Restriction recognition sites introduced for cloning purposes are underlined. All primers were designed on the basis of H. pylori 26695 genome nucleotide sequence.
1Underlined letters highlight engineered restriction enzyme (RE) sites (names of the introduced RE sites are incorporated in the primer names). Primer sequences.
