Tn-Seq analyses of Paraburkholderia kururiensis M130 and Burkholderia vietnamiensis LMG10929 during rice root colonization (cv japonica Nipponbare and cv indica IR64)

In this study we produced Tn-Seq libraries (Mariner transposon) of Paraburkholderia kururiensis M130 and Burkholderia vietnamiensis LMG10929 and tested them in broth medium control condition (Lubria Broth low salt medium to establish essential genes bacterial repertoires) versus rice root colonization to assess plant colonization essential genes(on cultivar japonica Nipponbare and cultivar indica IR64 ; at 7 days post inoculation; in an hydroponic growth Magenta pot system in Hoagland medium diluted 6 times).