MIR214

microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA (pre-miRNA), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star (miRNA*) products. The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. The RefSeq represents the predicted microRNA stem-loop. [provided by RefSeq, Sep 2009]

microRNAs（miRNAs）是一种长度为20-24个核苷酸的非编码RNA，在多细胞生物中，miRNAs通过影响mRNA的稳定性和翻译来参与转录后基因表达的调控。miRNAs由RNA聚合酶II转录，作为带有帽子和多聚腺苷酸化的初级转录本（pri-miRNAs）的一部分，这些初级转录本可以是编码蛋白质或非编码的。初级转录本被Drosha核糖核酸酶III酶切割，产生一个约70个核苷酸的茎环状前体miRNA（pre-miRNA），该前体miRNA随后被细胞质中的Dicer核糖核酸酶进一步切割，生成成熟的miRNA和反义miRNA星形（miRNA*）产物。成熟的miRNA被整合到RNA诱导的沉默复合物（RISC）中，通过miRNA与靶mRNA的不完全碱基配对来识别靶mRNA，通常导致靶mRNA的翻译抑制或不稳定。RefSeq代表预测的microRNA茎环。[由RefSeq提供，2009年9月]