File includes Figures A—K.

Figure A. SDS-PAGE analysis of purified recombinant proteins, Figure B. Equilibrium titrations of hTRBPdenat and hPACTdenat with double-stranded siRNA, Figure C. Pre-steady state kinetic analyses of the association of hTRBPdenat or hPACTdenat and double-stranded siRNA, Figure D. Concentration dependencies of the first phase of double-strand siRNA binding (observed pseudo-first-order rate constant) with hTRBPdenat and hPACTdenat are shown, Figure E. Concentration dependencies of the first phase of double-strand siRNA binding (observed pseudo-first-order rate constant) with hTRBPnat and hPACTnat are shown, Figure F. Kinetics of the dissociation of hTRBPnat/ds-siRNA-complexes, Figure G. Kinetics of the dissociation of hTRBPdenat/- or hPACTdenat/ds-siRNA-complexes, Figure H. hAgo2-mediated cleavage of target RNA in presence or absence of dsRNA-binding proteins, Figure I. Detection of protein-protein interactions between hAgo2 and hPACT or hTRBP, Figure J. hAgo2-PAZ9-mediated RNA cleavage and Figure K. hAgo2-catalyzed RNA cleavage as a function of the concentration of dsRNA-binding proteins. (PDF)