Simultaneous profiling of sexually transmitted bacterial pathogens, microbiome, and concordant host response in cervical samples using whole transcriptome sequencing analysis

We examined the potential for characterization of host, pathogen and microbiome interactions at a molecular level and identification of novel, outcome-relevant biomarkers in a single, easily obtained, clinical specimen using total RNA-seq. Overall design: Examination of human cervical swabs via total RNA-seq CT = Chlamydia trachomatis GC = Neisseria gonorrhoeae MG = Mycoplasma genitalium TV = Trichomonas vaginalis