Disease/molecular findings in primary MPXV challenged prairie dogs (shaded) and secondarily infected prairie dogs.

Four primary challenged animals (highlighted in grey) were inoculated via intranasal route with 9×103 pfu (0.07XLD50) of West African MPXV and placed into one of three experimental groups with naive animals. The time-line shown is for day post infection of the primary challenged animal; for the naive bedding group numbers in parentheses are days post placement in the trough. Two times a week animals were anesthetized for sample collection, lesion count and weight measurements. Three of the naive animals (indicated by crosses) were euthanized due to extreme morbidity during the study.