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Concentration, purity and RNA integrity number (RIN) of isolated and diluted total RNA samples.

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Purity of RNA was evaluated based on absorbance ratios which should be approximately 2.0. Lower A260/A230 hints at the presence of organic contaminants, for example acidic phenol. RNA with sufficient integrity for all downstream applications is characterized by RIN 8.0 or more.

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2019-02-11
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