Lack of electron acceptors contributes to redox stress and growth arrest in asparagine-starved sarcoma cells

Adequate availability of the non-essential amino acid asparagine is necessary to support growth and survival of sarcoma cells. The three top pathways enriched among transcripts upregulated in asparagine-depleted cells included ribosome, aminoacyl tRNA biosynthesis and oxidative phosphorylation. Overall design: Transcriptional changes in asparagine-depleted mouse sarcoma cells compared to control mouse sarcoma cells were evaluated by RNA-Seq.