Additional file 2 of The intrinsically disordered region of GCE protein adopts a more fixed structure by interacting with the LBD of the nuclear receptor FTZ-F1

Additional file 2: Figure S2. GCEC purification. A) Preparative SEC performed on the Superdex 200 10/300 GL column. Fractions selected for SDS-PAGE are indicated by fraction numbers. Fractions containing purified GCEC are marked with the grey color. B) Commassie Brilliant Blue R 250-stained SDS-PAGE analysis of the GCEC samples. Lane 1, molecular mass standards; lane 2, refolded proteins; lane 3, proteins not bound to the Ni2+-NTA resin; lanes 4–5, fractions after elution; lane 6, aggregated GCEC protein; lanes 7–8, purified GCEC.