Signal peptide cleavage prediction for GP5 of all Arteriviruses.

Representative N-terminal sequences (1–50) of GP5 of each member of the Arterivirus genus: EAV, equine arteritis virus (strain Bucyrus, GenBank accession number [ABI64076.1], PRRSV: porcine reproductive and respiratory syndrome virus (type 1/European, strain Lelystad [AAA46278.1] and type 2/North American, strain VR 2332 [AAD12129.1]), LDV: murine lactate dehydrogenase-elevating virus (NCBI reference sequence [NP_042577.1]), SHFV: simian hemorrhagic fever virus (NCBI reference sequence [NP_203550.1]). The predicted signal peptide (in small letters) and the “D value” for the most probable cleavage site (vertical bar) according to bioinformatics prediction with SignalP 4.0 are indicated. (D is a measure for cleavage likelihood, threshold: 0.45– note that the signal peptide of SHFV is predicted not to be cleaved.) Potential N-glycosylation sites are highlighted in bold (proven experimentally for LDV [57]). For PRRSV type 2, two signal peptide cleavage sites are possible (indicated with vertical bar). The decoy epitope (VLAN, in italics) would be absent in the mature protein if cleavage is performed at the most likely site (A31|S32).