MicroRNA expression profile of porcine alveolar macrophages infected with Toxoplasma gondii

Purpose: Porcine alveolar macrophage was infected by T. gondii including Rh strain and Me49 strain. We want to explore the change of miRNAs after infected with T. gondii in porcine alveolar macrophages. Results: Our study generated six mi RNA expression profiles from macrophages which infect with Rh strain and Me49 and control group in different time. Compare with T. gondii-infected and uninfected with T. gondii, 81 differentially expressed mi RNAs were identified, including 36 novel mi RNAs and 45 mature mi RNAs. The sample were harvested at 12h and 24h after porcine alveolar macrophages (3D4-21) infected with Rh strain (Type I) and Me49 strain (Type II). MiRNA libraries were generated using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.) and miRNA expression levels were estimate by Transcript per million (TPM).