The Raw datasets of the study A novel two-component system MprA/B (MprA/B1) was involved in regulation of metabolism, antibiotics resistance and virulence in Nocardia seriolae

<em>Nocardia seriolae </em>is one of the most important bacterial pathogens in cultured fish, causing serious necrotic granulomas in tissues. As a Gram-positive bacterium belonging to the order Actinomycetales, the pathogenic mechanism of <em>N. seriolae </em>is complex and not well defined. Two-component systems (TCS) function as signal transducers in prokaryotes that respond to different environmental stimuli. In this study, a novel two-component system gene, <em>mprA/B</em> homolog (<em>mprA/B</em>1), was identified in <em>N. seriolae</em> and a double gene-deficient mutant was generated. The Δ<em>mprA/B</em>1 strain exhibited faster growth and a more turbid bacterial suspension in trypticase soy broth (TSB). Transmission electron microscopy revealed an extra irregular polysaccharide layer outside the cell wall in the Δ<em>mprA/B</em>1 strain. Moreover, the Δ<em>mprA/B</em>1 strain showed a significantly increased sensitivity to macrolides and rifampicin. In a cell line, both strains were able to invade and survive intracellularly and induce cell apoptosis, but the Δ<em>mprA/B</em>1 strain showed lower cell invasion and intracellular survival rates. In largemouth bass, the Δ<em>mprA/B</em>1 strain showed significantly less tissue bacterial load and attenuated virulence. RNA sequencing identified 492 upregulated and 851 downregulated genes in the Δ<em>mprA/B</em>1 strain, and the gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment showed that many upregulated genes were involved in sugar and amino acid metabolic processes, and many downregulated genes were involved in drug efflux pump and virulence factor. These findings provide functional insights into the role of MprA/B in <em>N. seriolae</em>.<br>

诺卡氏菌（Nocardia seriolae）是养殖鱼类中最重要的细菌性致病菌之一，可引发宿主组织出现严重的坏死性肉芽肿。作为隶属于放线菌目（Actinomycetales）的革兰氏阳性菌，诺卡氏菌的致病机制复杂且尚未完全阐明。双组分系统（Two-component systems, TCS）是原核生物中响应不同环境刺激的信号转导因子。本研究中，研究人员在诺卡氏菌中鉴定出一种新型双组分系统基因——mprA/B同源基因（mprA/B1），并构建了该基因的双缺失突变株。ΔmprA/B1突变株在胰蛋白胨大豆肉汤（trypticase soy broth, TSB）中生长速度更快，菌悬液浊度更高。透射电子显微镜观察显示，ΔmprA/B1突变株的细胞壁外侧多出一层不规则的多糖层。此外，ΔmprA/B1突变株对大环内酯类抗生素与利福平的敏感性显著升高。在细胞系实验中，两株菌均可侵入宿主细胞并在胞内存活，同时诱导细胞凋亡，但ΔmprA/B1突变株的细胞侵袭率与胞内存活率均更低。在大口黑鲈模型中，ΔmprA/B1突变株的组织载菌量显著降低，毒力减弱。RNA测序结果显示，ΔmprA/B1突变株中有492个基因上调表达、851个基因下调表达；基因本体（Gene Ontology, GO）与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）通路富集分析表明，上调基因多参与糖类与氨基酸代谢过程，而下调基因多与药物外排泵及毒力因子相关。本研究结果为阐明MprA/B双组分系统在诺卡氏菌中的功能提供了新的理论依据。

搜集汇总

背景与挑战

背景概述

该数据集提供了关于新型双组分系统MprA/B（MprA/B1）在鱼类病原菌Nocardia seriolae中调节代谢、抗生素抗性和毒力的原始实验数据。研究通过比较野生型和突变菌株，发现MprA/B1缺失影响细菌生长、抗生素敏感性、细胞侵袭能力和毒力，并包含RNA测序分析结果。数据集包含Excel格式的原始数据和RNA-Seq分析结果的压缩文件，适用于渔业科学和农业与兽医科学领域的研究。