Natural variation in the consequences of gene overexpression during osmotic stress [RNA-Seq]

We used RNA-Seq to measure transcript abundance in 4 Saccharomyces cerevisiae strains (BY4743, BC187, NCYC3290, and YPS128) from a diverse range of genetic lineages when growing in rich media (YPD) with 0.7M NaCl to characterize differential expression across strains in response to osmotic and ionic stress.本研究采用RNA测序（RNA-Seq）技术，对4株源自多样遗传谱系的酿酒酵母（Saccharomyces cerevisiae）菌株（BY4743、BC187、NCYC3290与YPS128）的转录本丰度进行测定。上述菌株在添加0.7M氯化钠的富培养基（YPD）中培养，以表征各菌株在渗透胁迫与离子胁迫响应过程中的差异表达模式。