<i>ABO</i> genotyping and detection of blood group antigens on MSCs.

The ABO[25], [26] and FUT2 (secretor) genotype of MSCs was determined with PCR, the ABH histo-blood group phenotype predicted accordingly, and expression of ABH antigens was detected with flow cytometry. The relative fluorescence intensity (RFI) for binding of anti-A, -B, and –H was calculated by dividing the median fluorescence intensity (MFI) obtained for cells labeled with the corresponding antibody, by the MFI obtained for cells labeled with secondary antibody only. Abbreviations: ABO-ASP, ABO allele-specific primer PCR; FUT2-ASP, fucosyltransferase 2 allele-specific primer PCR (nucleotide 428 polymorphism).