Fluorescent images of actin and DAPI-labelled MCF10A, MCF7 and MDA-MB-231 cell lines

This dataset of cell images was generated to understand the morphological changes between less and more metastic cancer cells and between normal and cancerous cells. They have been used in the linked publications., Please refer to the README file and the linked publications for details on how this dataset was collected., , # Fluorescent images of actin and DAPI-labelled MCF10A, MCF7 and MDA-MB-231 cell lines

[https://doi.org/10.5061/dryad.sbcc2frfv](https://doi.org/10.5061/dryad.sbcc2frfv)

## Description of the data and file structure

Cell Lines: MCF10A, MCF7 and MDA-MB-231 are commonly cultured cell lines for breast cancer studies. MCF10A is a non-tumorigenic human mammary gland epithelial cell line, which represents normal breast cells. MCF7 is perhaps the most widely studied breast cancer line. MDA-MB-231 is a triple negative breast cancer cell line, often used as a model for breast cancer metastasis. MCF10A was purchased from the American Type Culture Collection (ATCC, Manassas, VA), while MCF7 and MDA-MB-231 cells were gifted to us.

Culture Conditions: MCF10A cells were cultured in MEGM kit (Lonza/Clonetics Corporation) without the GA-1000 (gentamycin-amphotericin B mix) component and with 100 ng/ml cholera toxin. The other two cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (...