Transcriptomic analysis of barley (Hordeum vulgare) after infection by Pyrenophora teres

The barley disease-resistant material BYT-CYA3 (B) and the sensitive material Mei 41 / I (M) were treated with ethanol and treated with diluted sodium chlorate for 10 minutes. The seeds will then be rinsed with distilled water, and then the seeds will be germinated in a sterile petri dish with double-layer sterile filter paper. After one week, the seeds germinated and transplanted into pots. Five plants were planted in each pot, and six replicates were set. After the seedlings reached the two-leaf and one-core stage, a suspension of Reticulum pathogen strain (LQA) was sprayed. After the inoculation is complete, leave it in the dark