Shedding lights on the alamandine signaling in CHO-MrgD and human pancreatic carcinoma cells: an antiproliferative effect was unveiled

Antiproliferative effect of ang-(1-7) has been further described and detailed, assessing its possibility as a therapeutic cancer control agent. Other endogenous peptides in this system have been studied and characterized, such as alamandine, a heptapeptide very similar to ang-(1-7) in terms of amino acid sequence and physiological effects. In this study, we described for the first time the anti-proliferative effect induced by alamandine treatment in human cancer cells (Mia Paca-2 and A-549) and its signaling in CHO-MrgD by phosphoproteomics. Our results showed that alamandine inhibited cancer cell growth in vitro assays, but did not in non-cancer cells. Our proteomics results revealed that alamandine reprogramed the energetic metabolism in Mia Paca-2, inhibited PI3K/AKT/mTOR pathways and translocated Fox01 to nucleus portion, not only but also is able to decrease cell growth and possibly migration regulating several proteins related to actin cytoskeleton and focal adhesion. Based in our phosphoproteomics results of alamandine signaling in CHO-MrgD, we have unveiled the anti-proliferative mechanism induced by alamandine/MrgD interaction.