Effects of DNA topoisomerase inhibitor acriflavine on endothelial gene expression under hypoxic conditions

RNA-Seq of human endothelial cells treated with acriflavine (ACF) with or without hypoxia revealed massive changes on gene expression. These were non-randomly and potentially due to DNA topoisomerase inhibition rather than HIF inhibition. Surprisingly, in contrast to protein-coding genes, RNA-Seq yielded that an exceeding number of lncRNAs is upregulated. Overall design: Examination of acriflavine treatment in HUVECs under hypoxia (16h, 1% O2)