S6 Data -

S2 Fig. (A–C) Growth curves for V. campbellii BB120 strains in LM (A), M9G (B), or M9GC (C) media. (D) Variability in lag phase of HCD-locked DS40M4 mutants grown in M9G medium. Three additional biological replicates of experiments in Fig 1C are shown. (E, F) The difference in cfus/ml between time points 1 and 2 (F) of wild-type and luxO D47E DS40M4 strains grown in M9G (F). Error bars show the mean and standard deviation of 5 biological replicates. (G) Growth curves for V. campbellii DS40M4 strains in M9 medium supplemented with maltose (10 mM). (H–K) Growth curves shown for V. coralliilyticus (H, J) or V. cholerae (I, K) grown in M9G (H, I) or M9GC (J, K). (A–E, G–K) The y-axis represents cell density OD600. For all panels, the data are from a single experiment that is representative of at least 3 independent biological experiments for each strain under every condition. (XLSX)