ChIP-seq analysis of MED1 and FOSL1 in SCC1 cells after depletion of FOSL1

To explore genome-wide alteration MED1 and FOSL1 after depletion of FOSL1, we performed chromatin immunoprecipitation sequencing (ChIP-seq) of SCC1 cells to examine genome-wide recruitment of MED1 and FOSL1 following FOSL1 knockdown. Depletion of FOSL1 led to dramatically loss of the recruitment of MED1 and FOSL1 at a cohort of key oncogenes associate with tumorigenesis and metastasis. Overall design: Examination of ChIP-seq of HNSCC cells treated with FOSL1 siRNA.