Gene expression profile of control or shEphB6-depleted D2.0R-EGFP cells in monoculture or cocultured with AT1-like cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE162439
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We studied how crosstalk with AT1-like cells affects transcriptome of disseminated dormant breast cancer cells and the requirement of EphB6 expression in the cancer cells for this genetic program D2.0R-EGFP-shControl or D2.0R-EGFP-shEphB6 (1,8x105 cells/well) were plated on top of AT1-like cells (1,36x106 cells/sample) in MLNL medium (in 60 mm dish) for three days before separation by Fluorescence-Activated Cell Sorter (FACS) based on EGFP signal. EGFP-+cells (D2.0R cells) were subjected to analysis.
创建时间:
2021-04-20



